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Red cell investigations: Art and artefacts
- Publication Year :
- 2013
-
Abstract
- Red blood cell research is important for both, the clinical haematology, such as transfusion medicine or anaemia investigations, and the basic research fields like exploring general membrane physiology or rheology.Investigations of red blood cells include a wide spectrum of methodologies ranging from population measurements with a billion cells evaluated simultaneously to single-cell approaches. All methods have a potential for pitfalls, and the comparison of data achieved by different technical approaches requires a consistent set of standards.Here, we give an overview of common mistakes using the most popular methodologies in red blood cell research and how to avoid them. Additionally, we propose a number of standards that we believe will allow for data comparison between the different techniques and different labs. We consider biochemical analysis, flux measurements, flow cytometry, patch-clamp measurements and dynamic fluorescence imaging as well as emerging single-cell techniques, such as the use of optical tweezers and atomic force microscopy.
- Subjects :
- Diagnostic Imaging
Erythrocytes
Population
2720 Hematology
Optical tweezers
610 Medicine & health
Biology
Microscopy, Atomic Force
Fluorescence imaging
Atomic force microscopy
Basic research
Animals
Humans
education
education.field_of_study
Hematology
Flow Cytometry
10081 Institute of Veterinary Physiology
Oncology
10076 Center for Integrative Human Physiology
Biochemical analysis
Flux measurements
Biophysics
570 Life sciences
biology
2730 Oncology
Biochemical engineering
Patch-clamp
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....8fbaba7207a9c9ffa20936895f60c76e