Bioproduction, purification and physicochemical characterization of melanin from Streptomyces sp. strain MR28

Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC)