Back to Search
Start Over
Circ_0001438 participates in the pathogenesis of preeclampsia via the circ_0001438/miR-942/NLRP3 regulatory network
- Source :
- Placenta. 104
- Publication Year :
- 2020
-
Abstract
- Introduction Preeclampsia (PE) is a common pregnancy disorder with multisystem complications. The growing data suggest that circular RNAs (circRNAs) involve in the development of PE. This study proposed to investigate the function and potential mechanisms of circ_0001438 in PE. Methods The expression of circ_0001438, miR-942 and NOD-like receptor pyrin domain-containing protein 3 (NLRP3) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The expression at the protein level of NLRP3, interleukin 1 beta (IL-1β), interleukin 10 (IL-10), B-cell lymphoma 2 (Bcl-2), Cleaved-caspase-3 (Cleaved-casp-3), N-cadherin and E-cadherin was detected by Western blot. Cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and colony formation assay. Cell apoptosis was determined by flow cytometry assay. Cell migration and invasion were monitored by transwell assay. The target genes were obtained and verified by the online bioinformatics tool and dual-luciferase reporter assay. Results The expression of circ_0001438 and NLRP3 was enhanced in PE placenta tissues. Circ_0001438 knockdown promoted cell proliferation, migration and invasion but inhibited apoptosis and inflammatory responses in HTR-8/Svneo cells, and these effects were reversed by the inhibition of miR-942, a target of circ_0001438. Moreover, NLRP3 was bounded by miR-942. The enrichment of miR-942 accelerated cell proliferation, migration and invasion but depleted apoptosis and inflammatory responses, while these impacts were partly abolished by NLRP3 overexpression. Discussion Circ_0001438 sponged miR-942 to regulate the expression of NLRP3, and circ_0001438 aggravated the dysfunctions of human villous trophoblasts by mediating the miR-942/NLRP3 axis at least in part.
- Subjects :
- 0301 basic medicine
Adult
Apoptosis
Flow cytometry
Cell Line
03 medical and health sciences
0302 clinical medicine
Pre-Eclampsia
Cell Movement
Pregnancy
NLR Family, Pyrin Domain-Containing 3 Protein
medicine
Humans
Gene Regulatory Networks
Receptor
Cell Proliferation
Gene knockdown
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Cell growth
Chemistry
Obstetrics and Gynecology
Cell migration
RNA, Circular
Molecular biology
Trophoblasts
Interleukin 10
MicroRNAs
030104 developmental biology
Reproductive Medicine
Female
Developmental Biology
Pregnancy disorder
Subjects
Details
- ISSN :
- 15323102
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Placenta
- Accession number :
- edsair.doi.dedup.....8fa8533a21b82a43b31790e714640a34