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Potential use of N-carboxyethylchitosan in biomedical applications: Preparation, characterization, biological properties
- Source :
- International journal of biological macromolecules. 149
- Publication Year :
- 2019
-
Abstract
- N-carboxyethylchitosan (CECS) was successfully prepared via Michael addition reaction of chitosan (CS) with acrylic acid in water. The structure of CECS was characterized by Fourier transform Infra-Red spectrometry (FT-IR), 1HNMR, elemental analysis, X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC). Antibacterial activity of CECS was evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by using minimum inhibition concentration (MIC). The results showed that the prepared CECS soluble in water at wide range of pH values. In addition, it has amorphous character improve its chemical reactivity than CS itself, in addition it has been showed stronger antibacterial activity than chitosan itself due to the presence of both -COOH and -NH2 groups and the CECS shows higher antibacterial activity towards S. aureus than E. coli. Finally, the cytotoxicity of CECS has been evaluated through Cell viability assay, which confirm that CECS is non-toxic and tissue compatible like CS.
- Subjects :
- Thermogravimetric analysis
Staphylococcus aureus
Cell Survival
Biocompatible Materials
02 engineering and technology
Microbial Sensitivity Tests
medicine.disease_cause
Biochemistry
Cell Line
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Differential scanning calorimetry
X-Ray Diffraction
Structural Biology
Spectroscopy, Fourier Transform Infrared
medicine
Escherichia coli
Humans
Molecular Biology
030304 developmental biology
Acrylic acid
0303 health sciences
Calorimetry, Differential Scanning
Chemical modification
Water
General Medicine
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
chemistry
Solubility
cardiovascular system
Michael reaction
0210 nano-technology
Antibacterial activity
Nuclear chemistry
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....8fa6df65649a4b3c6e98f96c0e627735