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Genetic Susceptibility to Oral Cancer due to Combined Effects of GSTT1, GSTM1 and CYP1A1 Gene Variants in Tobacco Addicted Patients of Pashtun Ethnicity of Khyber Pakhtunkhwa Province of Pakistan
- Source :
- Asian Pacific Journal of Cancer Prevention. 16:1145-1150
- Publication Year :
- 2015
- Publisher :
- Asian Pacific Organization for Cancer Prevention, 2015.
-
Abstract
- Associations of GSTT1, GSTM1 and CYP1A1 gene variants with risk of developing oral cancer were evaluated in this study. A case-control study was conducted in Pashtun population of Khyber Pakhtunkhwa province of Pakistan in which 200 hospital based oral cancer cases and 151 population based healthy controls exposed to similar environmental conditions were included. Sociodemographic data were obtained and blood samples were collected with informed consent for analysis. GSTM1 and GSTT1 were analysed through conventional PCR method while specific RT-PCR method was used to detect CYP1A1 polymorphisms. Results were analyzed for conditional logistic regression model by SPSS version 20. The study shows that patients with either GSTM1 or GSTT1 null genotypes have significantly higher risk of oral cancer (adjusted odds (OR): (3.019 (1.861-4.898) and 3.011(1.865-4.862), respectively), which further increased when either one or both null genes were present in combination (adjusted odds (OR): (3.627 (1.981-6.642 and 9.261 (4.495-19.079), respectively). CYP1A1 rs4646903 gene variants individually showed weak association OR: 1.121 (0.717-1.752); however, in the presence of GSTM1 and/or GSTT1 null genotypes further increasing the association (adjusted odds (ORs): 4.576 (2.038-10.273), 5.593 (2.530-12.362) and 16.10 (3.854-67.260 for GSTM/GSTT null and CYP1A1 wild type, GSTM/GSTT either null and CYP1A1 variant alleles, and all 3 gene polymorphisms combinations, respectively). Our findings suggest that presence of GSTM1 and/or GSTT1 null genotypes along with variant alleles of CYP1A1 may be the risk alleles for oral cancer susceptibility in Pashtun population.
- Subjects :
- Adult
Male
Oncology
Cancer Research
medicine.medical_specialty
Genotype
Epidemiology
Population
Ethnic group
Odds
Young Adult
Risk Factors
Internal medicine
Cytochrome P-450 CYP1A1
Ethnicity
medicine
Genetic predisposition
Humans
Genetic Predisposition to Disease
Pakistan
education
Gene
Aged
Glutathione Transferase
Neoplasm Staging
Genetics
education.field_of_study
Polymorphism, Genetic
business.industry
Null (mathematics)
Public Health, Environmental and Occupational Health
Cancer
Tobacco Use Disorder
Middle Aged
Prognosis
medicine.disease
Case-Control Studies
Female
Mouth Neoplasms
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 15137368
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Asian Pacific Journal of Cancer Prevention
- Accession number :
- edsair.doi.dedup.....8fa508d0fdc606bfd591d70b3644ddab