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Elevated levels of endothelial-derived microparticles, and serum CXCL9 and SCGF-β are associated with unstable asymptomatic carotid plaques
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Nature Publishing Group, 2015.
-
Abstract
- Endothelial microparticles (EMPs) are released from dysfunctional endothelial cells. We hypothesised that patients with unstable carotid plaque have higher levels of circulating microparticles compared to patients with stable plaques and may correlate with serum markers of plaque instability and inflammation. Circulating EMPs, platelet MPs (PMPs) and inflammatory markers were measured in healthy controls and patients undergoing carotid endarterectomy. EMP/PMPs were quantified using flow cytometry. Bioplex assays profiled systemic inflammatory and bone-related proteins. Immunohistological analysis detailed the contribution of differentially-regulated systemic markers to plaque pathology. Alizarin red staining showed calcification. EMPs and PMPs were significantly higher in patients with carotid stenosis (≥70%) compared to controls, with no differences between asymptomatic vs symptomatic patients. Asymptomatic patients with unstable plaques exhibited higher levels of EMPs, CXCL9 and SCGF-β compared to those with stable plaques. CXCL9 and SCGF-β were detected within all plaques, suggesting a contribution to both localised and systemic inflammation. Osteopontin and osteoprotegerin were significantly elevated in the symptomatic vs asymptomatic group, while osteocalcin was higher in asymptomatic patients with stable plaque. All plaques exhibited calcification, which was significantly greater in asymptomatic patients. This may impact on plaque stability. These data could be important in identifying patients at most benefit from intervention.
- Subjects :
- Male
Pathology
medicine.medical_specialty
medicine.medical_treatment
Osteocalcin
Inflammation
Carotid endarterectomy
Systemic inflammation
Hematopoietic Cell Growth Factors
Asymptomatic
Chemokine CXCL9
Article
Osteoprotegerin
Cell-Derived Microparticles
medicine
Humans
Carotid Stenosis
Lectins, C-Type
Osteopontin
Aged
Aged, 80 and over
Endarterectomy, Carotid
Multidisciplinary
biology
business.industry
Endothelial Cells
Middle Aged
medicine.disease
biology.protein
Cytokines
Female
medicine.symptom
Inflammation Mediators
business
Calcification
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8f9b612055eb7367d1df28fe58f829b0
- Full Text :
- https://doi.org/10.1038/srep16658