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Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup

Authors :
Robert H. Heflich
Takafumi Kimoto
David P. Lovell
Daniel J Roberts
Stephen D. Dertinger
Leon F. Stankowski
Daishiro Miura
B. Bhaskar Gollapudi
Javed A. Bhalli
Leslie Recio
Source :
Environmental and Molecular Mutagenesis
Publication Year :
2021

Abstract

The rodent Pig‐a assay is a flow cytometric, phenotype‐based method used to measure in vivo somatic cell mutation. An Organization for Economic Co‐operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number 4.93). This article provides advice on best practices for designing and conducting rodent Pig‐a studies in support of evaluating test substance safety, with a focus on the rat model. Various aspects of assay conduct, including laboratory proficiency, minimum number of animals per dose group, preferred treatment and blood sampling schedule, and statistical analysis are described.

Details

ISSN :
10982280
Volume :
62
Issue :
3
Database :
OpenAIRE
Journal :
Environmental and molecular mutagenesis
Accession number :
edsair.doi.dedup.....8f979d14c500f49c002289aed7729062