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Enhanced anti-tumor effects of HPV16E7(49-57)-based vaccine by combined immunization with poly(I:C) and oxygen-regulated protein 150
- Source :
- Cancer epidemiology. 37(2)
- Publication Year :
- 2012
-
Abstract
- Background : It is well known that both heat shock protein (HSP) and Toll-like receptor (TLR)3 agonist polyinosinic:polycytidylic acid (poly(I:C)) are capable of promoting the antigen-specific immune responses. In the current study, we assessed whether the anti-tumor effects of the HPV16E7 49–57 -based vaccine can be elevated by combined applications of poly(I:C) and oxygen-regulated protein 150 (ORP150) in a mouse cervical cancer model. Methods : Recombinant mouse ORP150 and HPV E7 49–57 peptide were combined to passively form the ORP150–E7 49–57 complex under heat shock conditions. The effects of ORP150–E7 49–57 complex plus poly(I:C) adjuvant on lymphocyte proliferation and functional cytotoxic T cells were investigated by methyl thiazolyl tetrazolium (MTT), ELISPOT, and non-radioactive cytotoxicity assays. Finally, the complex's therapeutic anti-tumor effects with and without adjuvant therapy were observed in a tumor challenge experiment. Results : This combination vaccine approach significantly enhanced the proliferation of splenocytes and induced strong E7 49–57 -specific CTL responses. More importantly, the ORP150–E7 49–57 complex plus poly(I:C) vaccine format demonstrated more potent anti-tumor effects than ORP150–E7 49–57 complex alone or E7 49–57 plus poly(I:C) in TC-1 tumor-bearing mice. Conclusion : Both poly(I:C) and ORP150 chaperone can synergistically enhance the anti-tumor effects of the HPV16E7 49–57 -based vaccine in vitro and in vivo. This strategy provides a platform for the design of a tumor therapeutic vaccine capable of inducing an effective anti-tumor immune response.
- Subjects :
- Cancer Research
Epidemiology
medicine.medical_treatment
Papillomavirus E7 Proteins
Uterine Cervical Neoplasms
Lymphocyte proliferation
Lymphocyte Activation
Cancer Vaccines
chemistry.chemical_compound
Mice
Immune system
In vivo
Interferon
Heat shock protein
medicine
Cytotoxic T cell
Animals
Humans
HSP70 Heat-Shock Proteins
Vaccines, Combined
Human papillomavirus 16
business.industry
Papillomavirus Infections
Vaccination
Proteins
Neoplasms, Experimental
Molecular biology
Xenograft Model Antitumor Assays
Peptide Fragments
Mice, Inbred C57BL
Disease Models, Animal
Poly I-C
Oncology
chemistry
Polyinosinic:polycytidylic acid
Cancer research
Female
business
Adjuvant
medicine.drug
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 1877783X
- Volume :
- 37
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cancer epidemiology
- Accession number :
- edsair.doi.dedup.....8f95510b5cf4fd24a26460cce1f94205