Back to Search
Start Over
In vivo striatal measurement of hydroxytyrosol, and its metabolite (homovanillic alcohol), compared with its derivative nitrohydroxytyrosol
- Source :
- Neuroscience letters. 579
- Publication Year :
- 2014
-
Abstract
- Phenolic compounds were measured by in vivo brain microdialysis in rat striatum. Basal extracellular levels of hydroxytyrosol, homovanillic alcohol and nitro-hydroxytyrosol were not detectable by HPLC with electrochemical detection. However, systemic administration of hydroxytyrosol (20 and 40 mg/kg, i.p.) showed a clear increase in the extracellular level of this compound. This increase was accompanied by an increase in the extracellular level of homovanillic alcohol, a metabolite of hydroxytyrosol formed by catechol-O-methyltransferase activity. Perfusion of hydroxytyrosol (20 μM) through the microdialysis cannula also produced an increase in the extracellular level of homovanillic alcohol. Systemic administration of nitro-hydroxytyrosol (20 and 40 mg/kg, i.p.) produced a small increase in the extracellular level of this compound. Our data show that hydroxytyrosol is a more brain penetrant phenolic compound than nitro-hydroxytyrosol. Accordingly, there is high cerebral metabolism of hydroxytyrosol to produce homovanillic alcohol by catechol-O-methyltransferase activity, that is saturated at the higher administered dose of hydroxytyrosol.
- Subjects :
- Male
Microdialysis
General Neuroscience
Metabolite
Homovanillic Acid
Pharmacology
Phenylethyl Alcohol
Catechol O-Methyltransferase
High-performance liquid chromatography
Rats
Neostriatum
chemistry.chemical_compound
chemistry
Biochemistry
In vivo
Extracellular
Systemic administration
Hydroxytyrosol
Animals
Rats, Wistar
Perfusion
Biotransformation
Subjects
Details
- ISSN :
- 18727972
- Volume :
- 579
- Database :
- OpenAIRE
- Journal :
- Neuroscience letters
- Accession number :
- edsair.doi.dedup.....8f7aef34f3d26d87a11ccaed07cfc281