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Expression of co-stimulatory molecules, chemokine receptors and proinflammatory cytokines in dendritic cells from normal and chronically inflamed rat testis
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2007
- Publisher :
- Oxford University Press, 2007.
-
Abstract
- The presentation of self antigens by dendritic cells (DC) plays an important role in the initiation and maintenance of autoimmunity. In a model of experimental autoimmune orchitis (EAO), we have previously characterized dominant testicular autoantigens and shown an increase in DC numbers during the course of disease. In this study, we have developed a protocol for the isolation of a highly pure population of DC (∼97%) from the testis of EAO and control rats to analyse the expression of major histocompatibility complex (MHC) class II and co-stimulatory molecules (CD80, CD86), chemokine receptors (CCR2, CCR7) and cytokines (IL-10, IL-12p70, TNF-α). By flow cytometry, we observed similar percentage and intensity levels of MHC class II, CD80 and CD86 expression in testicular DC in all groups. Moreover, by real-time RT-PCR we have detected significantly higher CCR7 mRNA level in isolated testicular DC from rats with EAO compared to controls, whereas the expression of CCR2 was decreased in orchitis. Transcripts of IL-12p40 were observed in DC from all groups, whereas the expression of IL-10 and the rate limiting IL-12 subunit p35 were detectable exclusively in testicular DC from the inflamed testes. In co-culture experiments, testicular DC isolated from EAO animals significantly enhanced naïve T-cell proliferation compared with control DC. Taken together these results suggest that testicular DC in control testis is not mature and functionally tolerogenic, whereas in EAO testis, IL-12 expression and stimulation of T-cell proliferation points to a mature immunogenic state prior imminent migration to the lymph nodes to amplify immune responses against testicular antigens. Fil: Rival, Claudia. Universidad de Buenos Aires; Argentina Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: von Wulffen, Werner. University of Giessen Lung Center; Alemania Fil: Hackstein, Holger. Institute for Clinical Immunology and Transfusion Medicine; Alemania Fil: Schneider, Eva. Justus Liebig Universitat Giessen; Alemania Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Meinhardt A.. Justus Liebig Universitat Giessen; Alemania Fil: Fijak, Monika. Justus Liebig Universitat Giessen; Alemania
- Subjects :
- Male
Embryology
CCR2
Chemokine
T-Lymphocytes
Gene Expression
C-C chemokine receptor type 7
TESTICULAR INFLAMMATION
Major Histocompatibility Complex
Chemokine receptor
Testis
EXPERIMENTAL AUTOIMMUNE ORCHITIS (EAO)
education.field_of_study
CO-STIMULATORY MOLECULES
biology
Interleukin-12 Subunit p40
Reverse Transcriptase Polymerase Chain Reaction
Obstetrics and Gynecology
Flow Cytometry
Interleukin-12
Spermatozoa
Interleukin-10
B7-1 Antigen
Cytokines
Receptors, Chemokine
purl.org/becyt/ford/3 [https]
medicine.medical_specialty
Receptors, CCR7
Receptors, CCR2
Population
purl.org/becyt/ford/3.5 [https]
DENDRITIC CELLS
Proinflammatory cytokine
Internal medicine
Genetics
medicine
Animals
Rats, Wistar
education
Molecular Biology
Cell Proliferation
Inflammation
MHC class II
Tumor Necrosis Factor-alpha
CYTOKINES
Cell Biology
Dendritic cell
Dendritic Cells
Molecular biology
Rats
Endocrinology
Reproductive Medicine
biology.protein
B7-2 Antigen
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Accession number :
- edsair.doi.dedup.....8f77a7a0ec9ace66587b9d9fcf505c9c