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Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production
- Source :
- Molecular Microbiology. 14:691-703
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Summary Flagellar hooks were purified from Helicobacter pylori and Helicobacter mustelae. The 70 × 16nm H. pylori hook was composed of FIgE subunits of 78kDa, while the 72 × 16nm H. mustelae hook was composed of 87kDa subunits. N-terminal sequence was obtained for the FIgH proteins of both species, and for an internal H. mustelae FlgE peptide. Degenerate oligonucleotide primers allowed amplification of a 1.2 kb fragment from the H. mustelae chromosome, which carried part of the flgE gene. The corresponding H. pylori gene was cloned by immunoscreening of a genomic library constructed in λZAP Express, The translated H. pylori flgE sequence indicated a protein with limited homology with the hook proteins from Salmonella typhimurium and Treponema phagedenis. Mutants of H. pylori and H. mustelae defective in hook production generated by allele replacement were non-motile and devoid of flagellar filaments but produced both flagellin subunits, which were localized in the soluble fraction of the cell. The level of flagellin production was unchanged in the mutants, indicating that the regulation of flagellin expression in Helicobacter differs from that in the Enterobacteriaceae.
- Subjects :
- DNA, Bacterial
Molecular Sequence Data
Restriction Mapping
Mutant
Microbiology
Homology (biology)
Bacterial Proteins
Cell Movement
Helicobacter
Immunoscreening
Genomic library
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Gene
DNA Primers
Base Sequence
Helicobacter pylori
Sequence Homology, Amino Acid
biology
Gene Amplification
biology.organism_classification
Enterobacteriaceae
Molecular Weight
Microscopy, Electron
Flagella
Genes, Bacterial
Mutation
biology.protein
bacteria
Flagellin
Subjects
Details
- ISSN :
- 13652958 and 0950382X
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi.dedup.....8f632c6108894dc298c69ed41344d390