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The human Cx40 promoter polymorphism -44G--A differentially affects transcriptional regulation by Sp1 and GATA4
- Source :
- Biochimica et biophysica acta. 1759(10)
- Publication Year :
- 2006
-
Abstract
- Expression of the tissue-specific gap junction protein connexin(Cx)40 is regulated by the interaction of ubiquitous and tissue-specific factors such as Sp1 and GATA4. Cardiac Cx40 expression is altered under pathological conditions such as atrial fibrillation. A human promoter polymorphism, a G → A change at position − 44 that has been associated with atrial-specific arrhythmias, is located between the TBE-NKE-Sp and GATA consensus transcription factor binding sites important for the regulation of the mouse Cx40 gene. The presence of the A-allele at position − 44 in promoter–reporter constructs significantly reduces promoter activity. Using electrophoretic mobility shift assays and luciferase reporter assays in various cell types, we show that Sp1 and GATA4 are important regulators of human Cx40 gene transcription and that the − 44 G → A polymorphism negatively affects the promoter regulation by the transcription factors Sp1 and GATA4.
- Subjects :
- Sp1 Transcription Factor
Response element
Biophysics
E-box
Biology
Biochemistry
Connexins
Mice
Structural Biology
Genetics
Animals
Humans
Enhancer
Promoter Regions, Genetic
Transcription factor
DNA Primers
Polymorphism, Genetic
General transcription factor
Base Sequence
Promoter
TCF4
Molecular biology
GATA4 Transcription Factor
Gene Expression Regulation
cardiovascular system
GATA transcription factor
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 1759
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....8f589382df70003c2696168a9d389577