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Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia
- Source :
- BMC Genomics
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Background Blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction. Results Representative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins. Conclusions This research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-1466-8) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
Arthropod Antennae
Bioinformatics
Odorant binding
Molecular Sequence Data
Genome, Insect
Receptors, Cell Surface
Olfaction
Biology
Receptors, Odorant
Proteomics
Transcriptome
Genetics
Animals
Chemosensory proteins
Amino Acid Sequence
Receptor
Phylogeny
Odorant receptors
Diptera
Membrane Proteins
biology.organism_classification
Calliphora stygia
Smell
Membrane protein
Blowfly olfaction
Insect Proteins
Drosophila
Female
Sequence Alignment
Research Article
Biotechnology
Ionotropic effect
Subjects
Details
- ISSN :
- 14712164
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....8f4ea659cd314177e266013e456b7699