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Fabrication and in vitro evaluation of a packed-bed bioreactor based on an optimum two-stage culture strategy
- Source :
- Journal of bioscience and bioengineering. 127(4)
- Publication Year :
- 2018
-
Abstract
- A packed-bed (PB) bioreactor for bioartificial liver (BAL) was fabricated based on an optimum two-stage culture strategy and evaluated in vitro in this research. Human induced hepatocytes (hiHeps) were first expanded using Cytodex 3 microcarriers and the choice of microcarrier concentration and fetal bovine serum (FBS) content was optimized. Then, the cells expanded under the optimum expansion condition were perfused into a perfusion system containing Fibra-Cel (FC) disks to fabricate a PB bioreactor. Operating parameters including flow rate and seeding density for perfusion culture were optimized, respectively. Results indicated that during suspension culture, rapid cell proliferation and favorable amino acid metabolism were achieved at 3 mg/mL microcarriers combined with 1% FBS. While for the perfusion culture, the most effective flow rate and seeding density were 2 mL/min and 1 × 106 cells/mL, respectively. Under this optimum perfusion condition, hiHeps showed good proliferation ability, high viability, homogeneous distribution, high metabolism activities and efficient albumin secretion as well as high liver-specific genes expression. Therefore, the two-stage culture strategy based on operating parameters optimization provides a new method for the development of PB bioreactors.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cell Culture Techniques
Bioengineering
Cell Count
01 natural sciences
Applied Microbiology and Biotechnology
law.invention
03 medical and health sciences
Perfusion Culture
Bioreactors
law
010608 biotechnology
Manufacturing Industry
Bioreactor
Humans
Cell Shape
Cells, Cultured
Packed bed
Chromatography
Chemistry
Albumin
Bioartificial liver device
Microcarrier
Dextrans
Equipment Design
Liver, Artificial
Perfusion
030104 developmental biology
Liver
Hepatocytes
Seeding
Fetal bovine serum
Biotechnology
Subjects
Details
- ISSN :
- 13474421
- Volume :
- 127
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of bioscience and bioengineering
- Accession number :
- edsair.doi.dedup.....8f465b65a7d7c76311e4303246d4fcc6