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Identification of NH+4-regulated genes of Herbaspirillum seropedicae by random insertional mutagenesis
- Source :
- Archives of Microbiology. 187:379-386
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- Random mutagenesis using transposons with promoterless reporter genes has been widely used to examine differential gene expression patterns in bacteria. Using this approach, we have identified 26 genes of the endophytic nitrogen-fixing bacterium Herbaspirillum seropedicae regulated in response to ammonium content in the growth medium. These include nine genes involved in the transport of nitrogen compounds, such as the high-affinity ammonium transporter AmtB, and uptake systems for alternative nitrogen sources; nine genes coding for proteins responsible for restoring intracellular ammonium levels through enzymatic reactions, such as nitrogenase, amidase, and arginase; and a third group includes metabolic switch genes, coding for sensor kinases or transcription regulation factors, whose role in metabolism was previously unknown. Also, four genes identified were of unknown function. This paper describes their involvement in response to ammonium limitation. The results provide a preliminary profile of the metabolic response of Herbaspirillum seropedicae to ammonium stress.
- Subjects :
- Genetics
Transposable element
Reporter gene
Herbaspirillum
Nitrogen
Mutagenesis (molecular biology technique)
Gene Expression Regulation, Bacterial
General Medicine
Biology
Herbaspirillum seropedicae
biology.organism_classification
Biochemistry
Microbiology
Quaternary Ammonium Compounds
Insertional mutagenesis
Mutagenesis, Insertional
Genes, Bacterial
Gene expression
Molecular Biology
Gene
Subjects
Details
- ISSN :
- 1432072X and 03028933
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Archives of Microbiology
- Accession number :
- edsair.doi.dedup.....8f38f7280c063e2e203884f57dcd9f44