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The expression of omental 11β-HSD1 is not increased in severely obese women with metabolic syndrome
- Source :
- Obesity facts. 5(1)
- Publication Year :
- 2011
-
Abstract
- Objective: Plasma cortisol in obese subjects does not differ from that in normoweight subjects. Extra-adrenal cortisol production by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) can result in local hypercortisolemia. The aim of the present study was to examine the role of visceral hypercortisolemia in the development of metabolic syndrome in severe obesity. Methods: Eight lean women during hysterectomy (controls) and 19 severely obese women during bariatric surgery were studied, 8 without metabolic syndrome (OM– group) and 11 with it (OM+ group). Biopsies of omental and subcutaneous fat were performed in the severely obese women during surgery, but only omental biopsies in the controls. Expression of 11β-HSD1, glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) was evaluated using real-time PCR.Results: Omental 11β-HSD1 expression was different between groups (one-way ANOVA, p < 0.01). Post-hoc analysis revealed that mean omental 11β-HSD1 mRNA levels were higher in the OM– group compared to controls, whereas they were similar when comparing the OM+ group with lean controls. Expression of 11β-HSD1 in subcutaneous fat was not different between OM+ and OM– groups. GRα expression in omental fat did not differ among groups or between omental and subcutaneous fat in severely obese patients. An expression of GRβ was not detected.Conclusion: Contrary to our original hypothesis, omental 11β-HSD1 expression is not increased in the OM+ group.
- Subjects :
- Adult
medicine.medical_specialty
Health (social science)
Intra-Abdominal Fat
Hydrocortisone
medicine.medical_treatment
Biopsy
Subcutaneous Fat
Bariatric Surgery
Hysterectomy
Real-Time Polymerase Chain Reaction
Young Adult
Glucocorticoid receptor
Receptors, Glucocorticoid
Physiology (medical)
Internal medicine
11-beta-Hydroxysteroid Dehydrogenase Type 1
Medicine
Humans
RNA, Messenger
Metabolic Syndrome
Analysis of Variance
medicine.diagnostic_test
business.industry
Middle Aged
medicine.disease
Obesity
Obesity, Morbid
Hypercortisolemia
Endocrinology
Female
Metabolic syndrome
business
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Subjects
Details
- ISSN :
- 16624033
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Obesity facts
- Accession number :
- edsair.doi.dedup.....8f2dc0eb38525c27116a1bb345f5dfd9