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Highly Efficient Sex Chromosome Interchanges Produced By I-CreI Expression in Drosophila
- Publication Year :
- 2005
- Publisher :
- Copyright © 2005 by the Genetics Society of America, 2005.
-
Abstract
- The homing endonuclease I-CreI recognizes a site in the gene encoding the 23S rRNA of Chlamydomonas reinhardtii. A very similar sequence is present in the 28S rRNA genes that are located on the X and Y chromosomes of Drosophila melanogaster. In this work we show that I-CreI expression in Drosophila is capable of causing induced DNA damage and eliciting cell cycle arrest. Expression also caused recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located, presumably as a result of a high frequency of double-strand breaks in these regions. Approximately 20% of the offspring of males expressing I-CreI showed exceptional inheritance of X- and Y-linked markers, consistent with chromosome exchange at rDNA loci. Cytogenetic analysis confirmed the structures of many of these products. Exchange between the X and Y chromosomes can be induced in males and females to produce derivative-altered Y chromosomes, attached-XY, and attached-X chromosomes. This method has advantages over the traditional use of X rays for generating X-Y interchanges because it is very frequent and it generates predictable products.
- Subjects :
- Genetics
Male
Recombination, Genetic
I-CreI
X Chromosome
biology
Heterochromatin
Chromosome
Mitosis
DNA Restriction Enzymes
Balancer chromosome
Investigations
biology.organism_classification
Y chromosome
Molecular biology
Homing endonuclease
Drosophila melanogaster
Y Chromosome
biology.protein
Animals
Female
X chromosome
DNA Damage
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....8f2a3b8220774caa13d25377a014947a