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Metabolite Profiles of Icariin in Rat Feces, Bile and Urine by Ultraperformance Liquid-Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry
- Source :
- Journal of chromatographic science. 54(2)
- Publication Year :
- 2016
-
Abstract
- In this work, the metabolite profiles of icariin in rat feces, bile and urine were qualitatively investigated, and the possible metabolic pathways of icariin were subsequently proposed. After oral administration of icariin at a single dose of 100 mg/kg, rat biological samples were collected and pretreated. Then, these pretreated samples were detected by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). In all, 17 metabolites were identified in the biosamples. Of these, 5, including F8-F9 (icariside I), D3-D4 (isopentenyl alcohol-icaritin-3-O-rha-7-O-gluA) and N3 (1,3-isoprene icariside II), were to our knowledge reported for the first time. The results indicated that icariin was metabolized via desugarization, dehydrogenation, hydroxylation, demethylation and glucuronidation pathways in vivo. This study revealed the possible metabolite profiles of icariin in rats.
- Subjects :
- Male
Metabolite
Glucuronidation
Mass spectrometry
Tandem mass spectrometry
01 natural sciences
Analytical Chemistry
Hydroxylation
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Feces
0302 clinical medicine
Tandem Mass Spectrometry
Animals
Bile
Chromatography, High Pressure Liquid
Demethylation
Epimedium
Flavonoids
Chromatography
biology
010401 analytical chemistry
General Medicine
biology.organism_classification
0104 chemical sciences
Rats
chemistry
Icariin
030217 neurology & neurosurgery
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 1945239X
- Volume :
- 54
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of chromatographic science
- Accession number :
- edsair.doi.dedup.....8f06ba67fa1b574408d214057bda20e2