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Candida albicans Germ-Tube Antibody: Evaluation of a New Automatic Assay for Diagnosing Invasive Candidiasis in ICU Patients
- Source :
- Mycopathologia. 182(7-8)
- Publication Year :
- 2016
-
Abstract
- Testing for Candida albicans germ-tube antibody IFA IgG assay (CAGTA) is used to detect invasive candidiasis infection. However, most suitable assays lack automation and rapid single-sample testing. The CAGTA assay was adapted in an automatic monotest system (invasive candidiasis [CAGTA] VirClia® IgG monotest (VirClia®), a chemiluminescence assay with ready-to-use reagents that provides a rapid objective result. CAGTA assay was compared with the monotest automatic VirClia® assay in order to establish the diagnostic reliability, accuracy, and usefulness of this method. A prospective study with 361 samples from 179 non-neutropenic critically ill adults patients was conducted, including 21 patients with candidemia, 18 with intra-abdominal candidiasis, 84 with Candida spp. colonization, and 56 with culture-negative samples, as well as samples from ten healthy subjects. Overall agreement between the two assays (CAGTA and VirCLIA) was 85.3%. These assays were compared with the gold-standard method to determine the sensitivity, specificity as well as positive and negative predictive values. In patients with candidemia, values for CAGTA and VirCLIA assays were 76.2 versus 85.7%, 80.3 versus 75.8%, 55.2 versus 52.9%, and 91.4 versus 94.3%, respectively. The corresponding values in patients with intra-abdominal candidiasis were 61.1 versus 66.7%, 80.3 versus 75.8%, 45.8 versus 42.9%, and 88.3 versus 89.3%, respectively. No differences were found according to the species of Candida isolated in culture, except for Candida albicans and C. parapsilosis, for which VirClia® was better than CAGTA. According to these results, the automated VirClia® assay was a reliable, rapid, and very easy to perform technique as tool for the diagnosis invasive candidiasis.
- Subjects :
- 0301 basic medicine
Icu patients
medicine.medical_specialty
Veterinary (miscellaneous)
030106 microbiology
Germ tube
Applied Microbiology and Biotechnology
Microbiology
Sensitivity and Specificity
03 medical and health sciences
Medical microbiology
Predictive Value of Tests
Positive predicative value
Candida albicans
medicine
Humans
Candidiasis, Invasive
Serologic Tests
Prospective Studies
Prospective cohort study
Antibodies, Fungal
Automation, Laboratory
Immunoassay
biology
Invasive candidiasis
medicine.disease
biology.organism_classification
Intensive Care Units
Immunoglobulin G
Immunology
Luminescent Measurements
biology.protein
Antibody
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 15730832
- Volume :
- 182
- Issue :
- 7-8
- Database :
- OpenAIRE
- Journal :
- Mycopathologia
- Accession number :
- edsair.doi.dedup.....8f00d09931a692e94724944a44275936