Back to Search
Start Over
The Mouse Thymosin Beta15 Gene Family Displays Unique Complexity and Encodes A Functional Thymosin Repeat
- Source :
- Journal of Molecular Biology. 387:809-825
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- We showed earlier that human beta-thymosin 15 (Tb15) is up-regulated in prostate cancer, confirming studies from others that propagated Tb15 as a prostate cancer biomarker. In this first report on mouse Tb15, we show that, unlike in humans, four Tb15-like isoforms are present in mouse. We used phylogenetic analysis of deuterostome beta-thymosins to show that these four new isoforms cluster within the vertebrate Tb15-clade. Intriguingly, one of these mouse beta-thymosins, Tb15r, consists of two beta-thymosin domains. The existence of such a repeat beta-thymosin is so far unique in vertebrates, though common in lower eukaryotes. Biochemical data indicate that Tb15r potently sequesters actin. In a cellular context, Tb15r behaves as a bona fide beta-thymosin, lowering central stress fibre content. We reveal that a complex genomic organization underlies Tb15r expression: Tb15r results from read-through transcription and alternative splicing of two tandem duplicated mouse Tb15 genes. Transcript profiling of all mouse beta-thymosin isoforms (Tb15s, Tb4 and Tb10) reveals that two isoform switches occur between embryonic and adult tissues, and indicates Tb15r as the major mouse Tb15 isoform in adult cells. Tb15r is present also in mouse prostate cancer cell lines. This insight into the mouse Tb15 family is fundamental for future studies on Tb15 in mouse (prostate) cancer models.
- Subjects :
- Male
Repetitive Sequences, Amino Acid
Gene isoform
Molecular Sequence Data
In Vitro Techniques
Biology
Mice
Species Specificity
Structural Biology
Transcription (biology)
Cell Line, Tumor
Gene Duplication
Biomarkers, Tumor
Animals
Humans
Protein Isoforms
Gene family
Amino Acid Sequence
Molecular Biology
Gene
Phylogeny
Actin
DNA Primers
Genomic organization
Genetics
Base Sequence
Sequence Homology, Amino Acid
Alternative splicing
Thymosin
Chromosome Mapping
Prostatic Neoplasms
Actins
Recombinant Proteins
Protein Structure, Tertiary
Rats
Multigene Family
NIH 3T3 Cells
Rabbits
hormones, hormone substitutes, and hormone antagonists
Protein Binding
Subjects
Details
- ISSN :
- 00222836
- Volume :
- 387
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Biology
- Accession number :
- edsair.doi.dedup.....8ec9264a923dd83ddc836097fffea968