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Ouabain Contributes to Kidney Damage in a Rat Model of Renal Ischemia-Reperfusion Injury
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 17, Iss 10, p 1728 (2016)
- Publication Year :
- 2016
- Publisher :
- MDPI, 2016.
-
Abstract
- Warm renal ischemia performed during partial nephrectomy has been found to be associated with kidney disease. Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI). We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase) and the protective effect of the ouabain inhibitor, rostafuroxin. We studied five groups of rats: (1) normal; (2) infused for eight weeks with ouabain (30 µg/kg/day, OHR) or (3) saline; (4) ouabain; or (5) saline-infused rats orally treated with 100 µg/kg/day rostafuroxin for four weeks. In group 1, 2–3 h after IRI, EO increased in ischemic kidneys while decreased in plasma. Nephrin progressively decreased and KIM-1 mRNA increased starting from 24 h. Ouabain infusion (group 2) increased blood pressure (from 111.7 to 153.4 mmHg) and ouabain levels in plasma and kidneys. In OHR ischemic kidneys at 120 h from IRI, nephrin, and KIM-1 changes were greater than those detected in the controls infused with saline (group 3). All these changes were blunted by rostafuroxin treatment (groups 4 and 5). These findings support the role of EO in IRI and suggest that rostafuroxin pre-treatment of patients before partial nephrectomy with warm ischemia may reduce IRI, particularly in those with high EO.
- Subjects :
- Kidney Disease
medicine.medical_treatment
Rostafuroxin
renal damage
030204 cardiovascular system & hematology
urologic and male genital diseases
Kidney
Nephrectomy
Ouabain
Catalysi
lcsh:Chemistry
Rats, Sprague-Dawley
0302 clinical medicine
lcsh:QH301-705.5
Saline
Spectroscopy
warm ischemia
Warm ischemia
biology
Chemistry
Communication
ouabain
Computer Science Applications1707 Computer Vision and Pattern Recognition
General Medicine
nephrin
Na-K ATPase
Computer Science Applications
medicine.anatomical_structure
030220 oncology & carcinogenesis
Reperfusion Injury
Kidney Diseases
medicine.drug
medicine.medical_specialty
Catalysis
Androstanol
Nephrin
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Renal damage
Animals
Androstanols
Na+/K+-ATPase
Physical and Theoretical Chemistry
Molecular Biology
Renal ischemia
Animal
urogenital system
Organic Chemistry
medicine.disease
Rats
rostafuroxin
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
Rat
Kidney disease
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 17
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....8ec34a686090867f4feaedb7dbf1d3b4