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Identification and expression analysis of the β-defensin genes in the goat small intestine
- Source :
- Gene. 801
- Publication Year :
- 2020
-
Abstract
- Defensins represent a family of cysteine-rich peptides that have broad-spectrum antimicrobial activities and serve as a typical kind of effector molecule in the immunity. Ruminant species have a large number of β-defensins in the absence of α- and θ-defensins. It is well-known that the genomes of sheep and cattle harbor at least 43 and 57 β-defensin genes, respectively. However, the repertoire of the goat β-defensin gene family has not been fully elucidated. In this study, we identified a total of 50 β-defensins from the goat genome, including 48 functional genes and 2 pseudogenes. Cross-species genomic and evolutionary analyses showed that all of the β-defensins in goat chromosomes 8, 13 and 23 present one-to-one orthologous relationships to their sheep and cattle counterparts, whereas some β-defensin genes in goat chromosome 27 are goat-specific. Moreover, we observed that some duplicated genes in goat chromosome 27 may be derived from gene copy number variation, and the annotation of sheep and cattle β-defensins appears to be incomplete in the genome. Importantly, real-time PCR analysis showed that 17 β-defensins are expressed in the small intestine with abundant cBD1s expression. These findings significant increased our knowledge of ruminant β-defensin and provided useful information for genetic studies, as well as providing a foundation for future research exploring the role of defensins in the immune response.
- Subjects :
- beta-Defensins
DNA Copy Number Variations
Pseudogene
Gene Expression
Biology
Real-Time Polymerase Chain Reaction
Genome
Chromosomes
Evolution, Molecular
Cathelicidins
Intestine, Small
Genetics
Gene family
Animals
Copy-number variation
Gene
Defensin
Phylogeny
Sheep
integumentary system
Effector
Goats
fungi
Chromosome
hemic and immune systems
General Medicine
respiratory system
bacterial infections and mycoses
Multigene Family
Cattle
Pseudogenes
Antimicrobial Cationic Peptides
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 801
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....8eb0294c964dadec20d3401e763b3000