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Anaphylactic bronchoconstriction in immunized guinea pigs provoked by inhalation and intravenous administration of hexahydrophthalic anhydride and methyltetrahydrophthalic anhydride
- Source :
- Allergy. 52:18-26
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- We established a guinea-pig model of anaphylactic bronchoconstriction provoked in immunized animals by inhalation and intravenous administration of 4,4-methyltetrahydrophthalic anhydride (MTHPA) and hexahydrophthalic anhydride (HHPA). Guinea pigs were immunized intradermally with either MTHPA (n = 8) or HHPA (n = 8) suspended in olive oil. Control animals (n = 8) were injected with olive oil alone. After 4 weeks, the animals were challenged during mechanical ventilation by inhalation or intravenous administration of MTHPA or HHPA conjugated with guinea-pig serum albumin (GPSA). Airway flow, and airway and esophageal pressures were measured. Resistance (R) and static compliance (Cst) of the respiratory system (rs), lung (1), and chest wall were studied with the flow-interruption technique. After challenge with MTHPA-GPSA or HHPA-GPSA, R,rs and R,1 increased dramatically while Cst,rs and Cst,1 decreased, and severe arterial hypoxia developed. The reaction occurred at a well-defined dose of anhydride and lasted about 30 min. When the same dose was repeated after 30 min, the response was much attenuated. MTHPA and HHPA can induce asthma in guinea pigs. The dose-response curve at antigen challenge is steep. Once a threshold dose is reached, a severe reaction occurs. The reactivity is then exhausted. This model may be suitable for assessing occupational asthma caused by acid anhydrides and possibly by other low-molecular-weight chemicals.
- Subjects :
- Male
Bronchoconstriction
Guinea Pigs
Immunology
Dose-Response Relationship, Immunologic
Pharmacology
Pulmonary compliance
Guinea pig
Administration, Inhalation
medicine
Animals
Immunology and Allergy
Respiratory system
Anaphylaxis
Aerosols
Inhalation
Epoxy Resins
business.industry
medicine.disease
Immunoglobulin G
Phthalic Anhydrides
Anesthesia
Injections, Intravenous
Toxicity
medicine.symptom
business
Occupational asthma
Subjects
Details
- ISSN :
- 13989995 and 01054538
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Allergy
- Accession number :
- edsair.doi.dedup.....8ea5d9fe650685b6b3e2cf44db14e596