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Screening of Crude Drugs Used in Japanese Kampo Formulas for Autophagy-Mediated Cell Survival of the Human Hepatocellular Carcinoma Cell Line
- Source :
- Medicines, Vol 6, Iss 2, p 63 (2019), Medicines, Volume 6, Issue 2
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Background: Autophagy is a catabolic process through which dysfunctional proteins and organelles are degraded, and that is associated with the proliferation of cancer cells. The aim of this study was to screen approximately 130 kinds of crude drugs used in Japanese Kampo formulas to identify crude drugs that would regulate the proliferation through autophagy of human hepatocellular carcinoma HepG2 cells. Methods: Extracts of each crude drug were prepared using methanol. Protein levels were determined using Western blotting. Cell viability was measured by MTT assay. Results: Among the 130 crude extracts, 24 of them increased LC3-II expression. Among these, Goboshi (burdock fruit), Soboku (sappan wood), Mokko (saussurea root), Rengyo (forsythia fruit), and Hikai (dioscorea) notably suppressed the proliferation of HepG2 cells and increased p62 expression levels, which suggested that these five extracts downregulate the autophagic activity resulting in the accumulation of p62. On the other hand, Hishinomi (water chestnut), Biwayo (loquat leaf), and Binroji (areca) induced cell growth and decreased or were uninvolved with p62 expression levels, which implied that these three extracts might induce autophagy modulators for cell growth. Conclusions: The results suggest that the compounds contained in the crude drugs selected for this study could control cell viability by regulating autophagic activity in HepG2 cells. The isolation and identification of the active compounds in these drugs might lead to the development of agents for autophagy research and cancer chemoprevention.
- Subjects :
- HepG2
Kampo
lcsh:Medicine
Pharmacology
Biology
Crude drug
Article
crude drugs
03 medical and health sciences
0302 clinical medicine
MTT assay
Viability assay
cell viability
030304 developmental biology
General Environmental Science
0303 health sciences
Cell growth
screening
p62
lcsh:R
Autophagy
autophagic activity
General Engineering
Blot
Kampo medicines
LC3-II
030220 oncology & carcinogenesis
Cancer cell
General Earth and Planetary Sciences
Subjects
Details
- ISSN :
- 23056320
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Medicines
- Accession number :
- edsair.doi.dedup.....8e8d0d39138cfe6d953a33f22cdb24c4