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Reduced Expression of Endothelial Connexin37 and Connexin40 in Hyperlipidemic Mice
- Source :
- Arteriosclerosis, Thrombosis, and Vascular Biology. 23:1391-1397
- Publication Year :
- 2003
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2003.
-
Abstract
- Objective— We sought to clarify the response of endothelial connexins to hyperlipidemia and lipid-lowering therapy. Methods and Results— Aortic endothelial gap junctions were analyzed by en face immunoconfocal microscopy and electron microscopy in C57BL/6 mice subjected to the following regimens: (1) normal chow (NC) for 3 months (3 mo), (2) NC for 9 mo, (3) NC for 3 mo, followed by a cholesterol-enriched diet (CED) for 6 mo, (4) NC for 3 mo and CED for 6 mo, with simvastatin in the final week, and (5) (in apoprotein E [apoE]-deficient mice) NC and examined at 3 mo and 7 to 9 mo. In wild-type mice, connexin37 (Cx37) and Cx40 were markedly downregulated in the CED-fed animals compared with those fed NC (CED vs 9-mo NC, 77% reduction in Cx37 and 65% reduction in Cx40; both P P P Conclusions— Mouse aortic endothelial gap junctions and connexins are downregulated during long-term hyperlipidemia. Short-term treatment with simvastatin leads to recovery of Cx37 expression but not Cx40 expression.
- Subjects :
- Male
Apolipoprotein E
Simvastatin
medicine.medical_specialty
Ratón
medicine.medical_treatment
Down-Regulation
Connexin
Hyperlipidemias
Connexins
Mice
Internal medicine
Hyperlipidemia
medicine
Animals
Tissue Distribution
Microscopy, Immunoelectron
Aorta
Chemotherapy
biology
Chemistry
Gap Junctions
medicine.disease
Mice, Inbred C57BL
Endothelial stem cell
Endocrinology
Enzyme inhibitor
biology.protein
Female
Endothelium, Vascular
Cardiology and Cardiovascular Medicine
medicine.drug
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....8e89ab2400aeff7582c1396b5fa39e7c