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Primary Activation of Antigen-Specific Naive CD4+and CD8+T Cells following Intranasal Vaccination with Recombinant Bacteria
- Source :
- Infection and Immunity. 76:5817-5825
- Publication Year :
- 2008
- Publisher :
- American Society for Microbiology, 2008.
-
Abstract
- The primary activation of T-helper and T-cytotoxic cells following mucosal immunization with recombinantStreptococcus gordoniiwas studied in vivo by adoptive transfer of ovalbumin (OVA)-specific transgenic CD8+(OT-I) and CD4+(OT-II) T cells. A recombinant strain, expressing on the surface the vaccine antigen Ag85B-ESAT-6 fromMycobacterium tuberculosisfused to OVA T-helper and T-cytotoxic epitopes (peptides 323 to 339 and 257 to 264), was constructed and used to immunize C57BL/6 mice by the intranasal route. Recombinant, but not wild-type, bacteria induced OVA-specific CD4+and CD8+T-cell clonal expansion in cervical lymph nodes, lung, and spleen. OVA-specific CD4+and CD8+T-cell proliferation appeared first in cervical lymph nodes and later in the spleen, suggesting a possible migration of activated cells from the inductive site to the systemic district. A significant correlation between the percentages of CD4+and CD8+proliferating T cells was observed for each animal. The expression of CD69, CD44, and CD45RB on proliferating T lymphocytes changed as a function of the cell division number, confirming T-cell activation following the antigen encounter. These data indicate that intranasal immunization with recombinantS. gordoniiis capable of inducing primary activation of naive antigen-specific CD4+and CD8+T cells, both locally and systemically.
- Subjects :
- CD4-Positive T-Lymphocytes
Adoptive cell transfer
Ovalbumin
Immunology
Enzyme-Linked Immunosorbent Assay
Mice, Transgenic
Spleen
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Microbiology
Epitope
Mice
Antigen
medicine
Animals
Cytotoxic T cell
Administration, Intranasal
Cell Proliferation
biology
Vaccination
Streptococcus gordonii
T lymphocyte
Flow Cytometry
biology.organism_classification
Adoptive Transfer
Molecular biology
Mice, Inbred C57BL
Infectious Diseases
medicine.anatomical_structure
Microbial Immunity and Vaccines
Female
Parasitology
CD8
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....8e85d5380def5ada1128aaea0d1ee13f