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Effects of donor oocytes and culture conditions on development of cloned mice embryos
- Source :
- Molecular reproduction and development. 66(2)
- Publication Year :
- 2003
-
Abstract
- Mice have been successfully cloned from somatic and embryonic stem (ES) cells using the "Honolulu method." In the present study, different donor oocytes and different culture conditions were compared to evaluate the developmental potential of nuclear transfer embryos reconstructed with an inbred ES cell line HM-1. Oocytes were recovered from two different F1 donors B6D2F1 (C57BL/6 x DBA/2) and B6CBAF1 (C57BL/6 x CBA). There was no effect of oocyte origin on development of cloned embryos to the morulae/blastocyst stage (B6D2F1 44.1% vs. B6CBAF1 45.0%), and the transferred embryos could develop to term. Two culture conditions were compared to show their ability to support development to the morulae/blastocyst stage of reconstructed embryos with B6D2F1 oocytes. The total cell number in the cloned blastocysts cultured in M16 with 20% oxygen was much higher than that observed in CZB with 20% oxygen. Low oxygen concentration during culture of nuclear transfer embryos in CZB medium showed no beneficial effect on pre-implantation development, no embryos developed to term after transfer to surrogate mothers. Our results demonstrated that not only B6D2F1, but B6CBAF1 oocytes, can be used for nuclear transfer. M16 medium is superior for culture of nuclear transfer embryos and low oxygen concentration with CZB medium during culture shows no benefit on development of cloned embryos. (C) 2003 Wiley-Liss, Inc.
- Subjects :
- Male
Nuclear Transfer Techniques
Somatic cell
Zygote
Cloning, Organism
Biology
Cell Line
Andrology
Embryonic and Fetal Development
Mice
Pregnancy
Culture Techniques
Genetics
medicine
Animals
Blastocyst
Cells, Cultured
Low oxygen
Stem Cells
Embryo
Total cell
Cell Biology
Oocyte
Embryo Transfer
Embryo, Mammalian
Embryonic stem cell
Molecular biology
Culture Media
Mice, Inbred C57BL
medicine.anatomical_structure
Cell culture
Mice, Inbred DBA
embryonic structures
Mice, Inbred CBA
Oocytes
Female
Developmental Biology
Subjects
Details
- ISSN :
- 1040452X
- Volume :
- 66
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.doi.dedup.....8e7214d47b86e36d86522aec43dd6a62