Back to Search
Start Over
Insulin-like growth factor-1 inhibition of apoptosis is associated with increased expression of the bcl-xL gene product
- Source :
- Endocrinology. 138(3)
- Publication Year :
- 1997
-
Abstract
- Differentiated PC12 cells, which become dependent on the presence of growth factors in the media and die by apoptosis after several hours of serum deprivation, were used to test the ability of IGF-1 to regulate the endogenous levels of the death-suppressing protein Bcl-xL, IGF-1 was capable of preventing apoptosis of serum-deprived differentiated PC12 cells at physiological concentrations, with optimal results seen at 10(-8) M. Incubation with the hormone resulted in a significant increase of Bcl-x mRNA after 3-6 h incubation and a doubling of Bcl-xL protein levels by 24 h incubation. Our results show that the protective effect of IGF-1 in PC12 cells is associated with an up-regulation of Bcl-xL mRNA and protein levels.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
bcl-X Protein
Endogeny
Bcl-xL
Apoptosis
Biology
PC12 Cells
Gene product
Insulin-like growth factor
Endocrinology
Internal medicine
Proto-Oncogene Proteins
medicine
Animals
RNA, Messenger
Insulin-Like Growth Factor I
Incubation
Messenger RNA
Molecular biology
Rats
Alternative Splicing
Proto-Oncogene Proteins c-bcl-2
biology.protein
Hormone
Subjects
Details
- ISSN :
- 00137227
- Volume :
- 138
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....8e6fd94d685a4bd023d6174b83338b6a