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The RNA-binding protein SERBP1 interacts selectively with the signaling protein RACK1
- Source :
- Cellular Signalling. 35:256-263
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The RACK1 protein interacts with numerous proteins involved in signal transduction, the cytoskeleton, and mRNA splicing and translation. We used the 2-hybrid system to identify additional proteins interacting with RACK1 and isolated the RNA-binding protein SERBP1. SERPB1 shares amino acid sequence homology with HABP4 (also known as Ki-1/57), a component of the RNA spicing machinery that has been shown previously to interact with RACK1. Several different isoforms of SERBP1, generated by alternative mRNA splicing, interacted with RACK1 with indistinguishable interaction strength, as determined by a 2-hybrid beta-galactosidase assay. Analysis of deletion constructs of SERBP1 showed that the C-terminal third of the SERBP1 protein, which contains one of its two substrate sites for protein arginine N-methyltransferase 1 (PRMT1), is necessary and sufficient for it to interact with RACK1. Analysis of single amino acid substitutions in RACK1, identified in a reverse 2-hybrid screen, showed very substantial overlap with those implicated in the interaction of RACK1 with the cAMP-selective phosphodiesterase PDE4D5. These data are consistent with SERBP1 interacting selectively with RACK1, mediated by an extensive interaction surface on both proteins.
- Subjects :
- 0301 basic medicine
Protein-Arginine N-Methyltransferases
RNA Splicing
RNA-binding protein
Biology
Receptors for Activated C Kinase
Article
Substrate Specificity
Retinoblastoma-like protein 1
03 medical and health sciences
Protein splicing
Humans
Protein Isoforms
Protein Interaction Maps
PGRMC1
Binding Sites
Receptor for activated C kinase 1
RNA-Binding Proteins
RNA
Translation (biology)
Cell Biology
Molecular biology
Cyclic Nucleotide Phosphodiesterases, Type 4
Cell biology
Repressor Proteins
030104 developmental biology
Amino Acid Substitution
RNA splicing
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 08986568
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Cellular Signalling
- Accession number :
- edsair.doi.dedup.....8e6fc6d21e7ec937bdda6f2e224997c9