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Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads
- Source :
- ACS Applied Materials & Interfaces. 13:46260-46269
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S-linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.
- Subjects :
- Materials science
viruses
Glycopolymer
Chromatography, Affinity
Virus
Madin Darby Canine Kidney Cells
chemistry.chemical_compound
Dogs
Influenza A Virus, H1N1 Subtype
Limit of Detection
Polysaccharides
Influenza, Human
Animals
Humans
General Materials Science
Surface plasmon resonance
Infectivity
biology
Viral Vaccine
Surface Plasmon Resonance
Viral Load
Virology
Microspheres
HEK293 Cells
Carbohydrate Sequence
chemistry
Colloidal gold
biology.protein
Ultracentrifuge
Neuraminidase
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....8e3f8d924d497e8af5cd89db7a11a1f4