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Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function
- Source :
- Semin Cell Dev Biol
- Publication Year :
- 2022
- Publisher :
- eScholarship, University of California, 2022.
-
Abstract
- Basic neuroscience research employs numerous forms of antibodies as key reagents in diverse applications. While the predominant use of antibodies is as immunolabeling reagents, neuroscientists are making increased use of intracellular antibodies or intrabodies. Intrabodies are recombinant antibodies genetically encoded for expression within neurons. These can be used to target various cargo (fluorescent proteins, reporters, enzymes, etc.) to specific molecules and subcellular domains to report on and manipulate neuronal function with high precision. Intrabodies have the advantages inherent in all genetically encoded recombinant antibodies but represent a distinct subclass in that their structure allows for their expression and function within cells. The high precision afforded by the ability to direct their expression to specific cell types, and the selective binding of intrabodies to targets within these allows intrabodies to offer unique advantages for neuroscience research, given the tremendous molecular, cellular and morphological complexity of brain neurons. Intrabodies expressed within neurons have been used for a variety of purposes in basic neuroscience research. Here I provide a general background to intrabodies and their development, and examples of their emerging utility as valuable basic neuroscience research tools.
- Subjects :
- 1.1 Normal biological development and functioning
Computational biology
Biology
Plasmid
Article
Intrabody
Antibodies
law.invention
ScFv
Paediatrics and Reproductive Medicine
Immunolabeling
Recombinant antibodies
Substance Misuse
Live cell imaging
law
Underpinning research
Cell specific
Neurons
Recombinant
Neurosciences
Brain
Cell Biology
Neuron
Recombinant Proteins
Mental Health
Good Health and Well Being
nervous system
FingR
Neurological
biology.protein
Recombinant DNA
Nanobody
Neuroscience research
Biochemistry and Cell Biology
Drug Abuse (NIDA only)
Function (biology)
Developmental Biology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Semin Cell Dev Biol
- Accession number :
- edsair.doi.dedup.....8e3dd6206e26b7bcc8bbf9f2e9f0393b