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Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- Chronic wasting disease (CWD) is a highly contagious prion disease affecting captive and free-ranging cervid populations. CWD has been detected in United States, Canada, South Korea and, most recently, in Europe (Norway, Finland and Sweden). Animals with CWD release infectious prions in the environment through saliva, urine and feces sustaining disease spreading between cervids but also potentially to other non-cervids ruminants (e.g. sheep, goats and cattle). In the light of these considerations and due to CWD unknown zoonotic potential, it is of utmost importance to follow specific surveillance programs useful to minimize disease spreading and transmission. The European community has already in place specific surveillance measures, but the traditional diagnostic tests performed on nervous or lymphoid tissues lack sensitivity. We have optimized a Real-Time Quaking-Induced Conversion (RT-QuIC) assay for detecting CWD prions with high sensitivity and specificity to try to overcome this problem. In this work, we show that bank vole prion protein (PrP) is an excellent substrate for RT-QuIC reactions, enabling the detection of trace-amounts of CWD prions, regardless of prion strain and cervid species. Beside supporting the traditional diagnostic tests, this technology could be exploited for detecting prions in peripheral tissues from live animals, possibly even at preclinical stages of the disease.
- Subjects :
- 0301 basic medicine
Male
Risk
Wasting Disease
European community
Lymphoid Tissue
Prions
animal diseases
030106 microbiology
Prion strain
lcsh:Medicine
Disease
Article
Fluorescence
Prion Proteins
03 medical and health sciences
Feces
Disease spreading
Settore BIO/10 - Biochimica
medicine
Animals
Prion protein
Chronic
Saliva
lcsh:Science
Multidisciplinary
biology
Mesocricetus
Transmission (medicine)
Arvicolinae
Norway
Deer
Biological techniques
lcsh:R
Brain
Chronic wasting disease
biology.organism_classification
medicine.disease
Virology
Bank vole
030104 developmental biology
Wasting Disease, Chronic
Biological Assay
Female
lcsh:Q
Neuroscience
Reindeer
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8e33f647272b8c79b20e50b4432d8f29