Back to Search
Start Over
In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2020
- Publisher :
- MyJove Corporation, 2020.
-
Abstract
- Ischemia/reperfusion-derived myocardial dysfunction is a common clinical scenario in patients after cardiac surgery. In particular, the sensitivity of cardiomyocytes to ischemic injury is higher than that of other cell populations. At present, hypothermia affords considerable protection against an expected ischemic insult. However, investigations into complex hypothermia-induced molecular changes remain limited. Therefore, it is essential to identify a culture condition similar to in vivo conditions that can induce damage similar to that observed in the clinical condition in a reproducible manner. To mimic ischemia-like conditions in vitro, the cells in these models were treated by oxygen/glucose deprivation (OGD). In addition, we applied a standard time-temperature protocol used during cardiac surgery. Furthermore, we propose an approach to use a simple but comprehensive method for the quantitative analysis of myocardial injury. Apoptosis and expression levels of apoptosis-associated proteins were assessed by flow cytometry and using an ELISA kit. In this model, we tested a hypothesis regarding the effects of different temperature conditions on cardiomyocyte apoptosis in vitro. The reliability of this model depends on strict temperature control, controllable experimental procedures, and stable experimental results. Additionally, this model can be used to study the molecular mechanism of hypothermic cardioprotection, which may have important implications for the development of complementary therapies for use with hypothermia.
- Subjects :
- Cell Survival
General Chemical Engineering
Ischemia
Apoptosis
Pharmacology
Mitochondria, Heart
General Biochemistry, Genetics and Molecular Biology
Cell Line
Flow cytometry
Hypothermia, Induced
In vivo
medicine
Animals
Humans
Myocyte
Myocytes, Cardiac
Heart metabolism
Membrane Potential, Mitochondrial
Cardioprotection
Caspase 8
General Immunology and Microbiology
medicine.diagnostic_test
Caspase 3
business.industry
Myocardium
General Neuroscience
Temperature
Reproducibility of Results
Hypothermia
medicine.disease
Oxygen
Glucose
medicine.symptom
Reactive Oxygen Species
business
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....8e2ff6cf9ec2e1b9eda0a908325f1865