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Metabolic Engineering of Lignan Biosynthesis in Forsythia Cell Culture
- Source :
- Plant and Cell Physiology. 50:2200-2209
- Publication Year :
- 2009
- Publisher :
- Oxford University Press (OUP), 2009.
-
Abstract
- Lignans are a large class of secondary metabolites in plants, with numerous biological effects in mammals, including antitumor and antioxidant activities. Sesamin, the most abundant furofuran-class lignan in sesame seeds (Sesamum plants), is produced by the cytochrome P450 enzyme CYP81Q1 from the precursor lignan, pinoresinol. In contrast, Forsythia plants produce dibenzylbutyrolactone-class lignans, such as matairesinol, from pinoresinol via the catalysis of pinoresinol/lariciresinol reductase (PLR) and secoisolariciresinol dehydrogenase. Here we present the engineering of lignan biosynthesis in Forsythia cell suspension cultures for the development of an efficient production method of beneficial lignans. A suspension cell culture prepared from leaves of Forsythia koreana produced lignans, mainly pinoresinol and matairesinol glucosides, at levels comparable with that obtained from the leaves. In an attempt to increase the pinoresinol content in Forsythia, we generated a transgenic cell line overexpressing an RNA interference (RNAi) construct of PLR (PLR-RNAi). Down-regulation of PLR expression led to a complete loss of matairesinol and an accumulation of approximately 20-fold pinoresinol in its glucoside form in comparison with the non-transformant. Moreover, the Forsythia transgenic cells co-expressing CYP81Q1 and PLR-RNAi exhibited production of sesamin as well as accumulation of pinoresinol glucoside. These data suggest Forsythia cell suspension to be a promising tool for the engineering of lignan production. To the best of our knowledge, this is the first report on transgenic production of an exogenous lignan in a plant species.
- Subjects :
- Physiology
Cell Culture Techniques
Dioxoles
Plant Science
Lignans
Metabolic engineering
chemistry.chemical_compound
Transformation, Genetic
Forsythia
Glucoside
Gene Expression Regulation, Plant
Sesamin
Furans
Secoisolariciresinol dehydrogenase
Cells, Cultured
Matairesinol
Lignan
Molecular Structure
biology
Cell Biology
General Medicine
Plants, Genetically Modified
biology.organism_classification
Plant Leaves
stomatognathic diseases
chemistry
Pinoresinol
Biochemistry
RNA Interference
Genetic Engineering
Subjects
Details
- ISSN :
- 14719053 and 00320781
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi.dedup.....8e2221391bd87749759bbe3d00c2c8b2