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Controlled Intracellular Self-Assembly and Disassembly of 19F Nanoparticles for MR Imaging of Caspase 3/7 in Zebrafish
- Source :
- ACS Nano. 9:761-768
- Publication Year :
- 2014
- Publisher :
- American Chemical Society (ACS), 2014.
-
Abstract
- Compared to (1)H MRI, (19)F MRI provides higher selectivity but lower sensitivity. Therefore, the need to inject high doses of the (19)F probe to improve its sensitivity for in vivo diagnosis remains a challenge. A "smart" strategy is needed that could locally concentrate a low-dose (19)F probe while avoiding the fast transverse relaxation of the probes. Locally self-assembling and disassembling (19)F nanoparticles may be an optimal measure to achieve this goal. Herein, we report a dual-functional probe 1 for glutathione (GSH)-controlled self-assembly and subsequent caspase 3/7 (Casp3/7)-controlled disassembly of formed nanoparticles (i.e., 1-NPs). Consecutive assembly and disassembly of 1-NPs translate to "off" and "on" (19)F magnetic resonance (MR) signal states, respectively. Employing this smart strategy, we successfully used 1 for the consecutive detection of GSH and Casp3/7 activity in vitro and in cells and imaging Casp3/7 activity in cells and in zebrafish at low doses with a 14.1 T magnetic field.
- Subjects :
- Models, Molecular
Intracellular Space
Molecular Conformation
General Physics and Astronomy
Nanoparticle
Nanotechnology
Caspase 3
Fluorine-19 Magnetic Resonance Imaging
chemistry.chemical_compound
In vivo
medicine
Animals
Humans
General Materials Science
Zebrafish
Caspase 7
medicine.diagnostic_test
biology
General Engineering
Magnetic resonance imaging
Hep G2 Cells
Glutathione
biology.organism_classification
In vitro
chemistry
Biophysics
Nanoparticles
Intracellular
Subjects
Details
- ISSN :
- 1936086X and 19360851
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- ACS Nano
- Accession number :
- edsair.doi.dedup.....8ded626599fbed82cbf23071bf1adaa7