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Antihyperglycemic mechanism of M16209, an antidiabetic agent, in 3T3-L1 adipocytes
- Source :
- Life Sciences. 60:1821-1831
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- We investigated the effect of M16209 (1-(3-bromobenzo[b]furan-2-ylsulfonyl)hydantoin) on glucose transport and the insulin signaling system in mouse-derived 3T3-L1 adipocytes. When M16209 (30 and 100 microM) was added to 3T3-L1 adipocytes and preincubated for 24 hours, the uptake of 2-deoxy-D-[3H]-glucose (2-DG) after insulin stimulation was enhanced. This effect was seen when preincubation with M16209 was performed in the presence of 6 and 20 ng/ml insulin, but M16209 did not increase the response to 600 ng/ml insulin. M16209 (100 microM) did not interfere with (125)I-insulin binding or with tyrosine phosphorylation of the insulin receptor beta-subunit and IRS-1. M16209 (100 microM) also had no effect on the level of glucose transporter (GLUT1 and GLUT4) protein, but it promoted the translocation of intracellular GLUT4 to the plasma membrane. In contrast, M16209 had no effect on the translocation of GLUT1. In summary, M16209 enhanced 2-DG uptake by 3T3-L1 adipocytes. Insulin binding to its receptor, autophosphorylation of the insulin receptor beta-subunit, and tyrosine phosphorylation of IRS-1 were unaffected by M16209. However, translocation of GLUT4 from the intracellular pool to the plasma membrane was facilitated.
- Subjects :
- medicine.medical_specialty
Monosaccharide Transport Proteins
Insulin Receptor Substrate Proteins
Macromolecular Substances
medicine.medical_treatment
Glucose uptake
Muscle Proteins
CHO Cells
General Biochemistry, Genetics and Molecular Biology
Mice
chemistry.chemical_compound
Cricetinae
Internal medicine
Insulin receptor substrate
Adipocytes
medicine
Animals
Hypoglycemic Agents
Insulin
Phosphorylation
General Pharmacology, Toxicology and Pharmaceutics
Benzofurans
Glucose Transporter Type 1
Glucose Transporter Type 4
biology
Hydantoins
Autophosphorylation
Biological Transport
Tyrosine phosphorylation
3T3 Cells
General Medicine
Phosphoproteins
Receptor, Insulin
Insulin receptor
Glucose
Endocrinology
chemistry
biology.protein
Tyrosine
GLUT4
Signal Transduction
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....8dd4f5595f8687820887d94f9ce3f620