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Vaccination with UV-inactivated nodavirus partly protects European sea bass against infection, while inducing few changes in immunity
- Source :
- e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía, instname, e-IEO: Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía, Instituto Español de Oceanografía
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Developing viral vaccines through the ultraviolet (UV) inactivation of virus is promising technique since it is straightforward and economically affordable, while the resulting viruses are capable of eliciting an adequate antiviral immune response. Nodavirus (NNV) is a devastating virus that mainly affects European sea bass juveniles and larvae, causing serious economic losses in Mediterranean aquaculture. In this work, a potential vaccine consisting on UV-inactivated NNV (iNNV) was generated and administered to healthy juveniles of European sea bass to elucidate whether it triggers the immune response and improves their survival upon challenge. First, iNNV failed to replicate in cell cultures and its intraperitoneal administration to sea bass juveniles also failed to produce fish mortality and induction of the type I interferon (IFN) pathway, indicating that the NNV was efficiently inactivated. By contrast, iNNV administration induced significant serum non-specific antimicrobial activity as well as a specific antiviral activity and immunoglobulin M (IgM) titres against NNV. Interestingly, few changes were observed at transcriptional level in genes related to either innate or adaptive immunity, suggesting that iNNV could be modulating the immune response at protein or functional level. In addition, the iNNV vaccinated group showed improved survival, reaching a relative survival percentage of 57.9%. Moreover, challenged fish that had been vaccinated presented increased serum antibacterial, antiviral and IgM titres, as well as the higher transcription of mhc1a, ifn, isg15 and cd8a genes in brain, while in the headkidney the transcription of mhc1a, mhc2b and cd8a was down-regulated and mx, isg15 and tcrb was up-regulated. Although the UV-inactivated vaccine against NNV showed promising results, more effort should be addressed to improving this prophylactic method by increasing our understanding of its action mechanisms, thus enabling the mortality rate of NNV to be further reduced<br />Sí
- Subjects :
- 0301 basic medicine
Acuicultura
Immunology
Aquaculture
Adaptive Immunity
Biology
Virus
Cell-mediated cytotoxicity
Fish Diseases
03 medical and health sciences
RNA Virus Infections
Immune system
Immunity
Animals
Nodaviridae
Centro Oceanográfico de Murcia
Sea bass
European sea bass
Immune response
Inactivated vaccine
fish
Nodavirus
cell-mediated 51 cytotoxicity
Viral Vaccine
Vaccination
Brain
Viral Vaccines
04 agricultural and veterinary sciences
Head Kidney
vaccination
Acquired immune system
immunity
Virology
Immunity, Innate
UV
UV, inactivated vaccine, nodavirus, European sea bass, cell-mediated 51 cytotoxicity, immune response
030104 developmental biology
Immunoglobulin M
Vaccines, Inactivated
Interferon Type I
040102 fisheries
0401 agriculture, forestry, and fisheries
Bass
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía, instname, e-IEO: Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía, Instituto Español de Oceanografía
- Accession number :
- edsair.doi.dedup.....8d89e7db60a12c0d2d4e751880ee25cd