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UDP-galactose:globoside galactosyltransferase in murine kidney
- Source :
- Journal of Lipid Research, Vol 31, Iss 12, Pp 2227-2234 (1990)
- Publication Year :
- 1990
- Publisher :
- Elsevier, 1990.
-
Abstract
- There are increased levels of stage-specific embryonic antigens-3 and -1 (SSEA-3 and SSEA-1) globo-series glycolipids in male versus female DBA/2 and C57BL/6 kidneys, respectively. To determine what enzymatic steps may be responsible for these differences, the activity and properties of UDP-galactose:globoside galactosyltransferase were studied in male and female mouse kidney microsomes. This enzyme participates in the biosynthesis of galactosylgloboside, SSEA-3 glycolipid; the reaction product was identified by high performance thin-layer chromatography (HPTLC) immunostaining. In C57BL/6 mice, the specific activity of the enzyme, in the presence of CHAPS, was 2-fold greater in the male than that in the female. Optimum pH for the enzyme from both sexes was about 5.6, and Mn2+ was essential for maximal activity. Fifty percent of the male and female enzyme activity was lost after preincubating the microsomes for 1 min at 55 degrees C; thereafter, the enzyme from female microsomes had a slower rate of denaturation. The Km for globoside in presence of sodium cholate for both male and female was 0.035 mM, but it was approximately 2-fold greater for the female in presence of CHAPS. The enzyme in male and female microsomes was differentially activated by CHAPS and cholate. The results suggest the presence of an enzyme modulator in these membranes. In DBA/2 mice, the enzyme activity was about 2-fold greater in males than that in the female. The specific activity of the enzyme in the two strains was of a similar magnitude.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Male
Enzyme modulator
Detergents
QD415-436
Kidney
Biochemistry
Uridine Diphosphate Galactose
Mice
Endocrinology
Glycolipid
Chaps
Microsomes
Animals
chemistry.chemical_classification
Globosides
Globoside
biology
Cell Biology
Hydrogen-Ion Concentration
Galactosyltransferases
Molecular biology
Enzyme assay
Mice, Inbred C57BL
Kinetics
Enzyme
chemistry
Mice, Inbred DBA
Microsome
biology.protein
Female
Specific activity
Subjects
Details
- Language :
- English
- ISSN :
- 00222275
- Volume :
- 31
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....8d6a5ff3ec444e76dc849c80d9cd2bc7