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Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE
- Source :
- BIO-PROTOCOL, Bio Protoc
- Publication Year :
- 2018
- Publisher :
- Bio-Protocol, LLC, 2018.
-
Abstract
- The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system. Mild non-ionic detergent digitonin is used to solubilize labile protein super-and megacomplexes, which are then separated with first-dimension blue native polyacrylamide gel electrophoresis (1D-BN-PAGE). The digitonin derived protein complexes are further solubilized with stronger detergent, β-DM, and subsequently separated on an orthogonal 2D-BN-PAGE to release smaller protein subcomplexes from the higher-order supercomplexes. Here we describe a detailed method for 2D-BN-PAGE analysis of thylakoid protein complexes from Arabidopsis thaliana.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Chemistry
Strategy and Management
Mechanical Engineering
ta1183
Metals and Alloys
food and beverages
Native Polyacrylamide Gel Electrophoresis
macromolecular substances
Native page
biology.organism_classification
01 natural sciences
Industrial and Manufacturing Engineering
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Digitonin
Biochemistry
Solubilization
Thylakoid
Methods Article
Arabidopsis thaliana
010606 plant biology & botany
Subjects
Details
- ISSN :
- 23318325
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- BIO-PROTOCOL
- Accession number :
- edsair.doi.dedup.....8d65f21ead0bc448cbed2685c345af57