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Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells
- Source :
- Biomedical research (Tokyo, Japan). 29(3)
- Publication Year :
- 2008
-
Abstract
- Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin alpha5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin alpha3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation.
- Subjects :
- Human leukemia
Base Sequence
Cell
Gene Expression
HL-60 Cells
Tretinoin
General Medicine
Importin
Biology
Karyopherins
environment and public health
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
Cell biology
medicine.anatomical_structure
Calcitriol
Leukemia, Promyelocytic, Acute
Nucleocytoplasmic Transport
Gene expression
Cancer research
medicine
Humans
DNA microarray
DNA Primers
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 1880313X
- Volume :
- 29
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedical research (Tokyo, Japan)
- Accession number :
- edsair.doi.dedup.....8d5d22db21ce29bf7c5c8ca25c6cf985