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Genetic diversity and population structure of the human malaria parasite Plasmodium falciparum surface protein Pfs47 in isolates from the lowlands in Western Kenya
- Source :
- PloS one, vol 16, iss 11, PLoS ONE, Vol 16, Iss 11, p e0260434 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- eScholarship, University of California, 2021.
-
Abstract
- Plasmodium falciparum parasites have evolved genetic adaptations to overcome immune responses mounted by diverse Anopheles vectors hindering malaria control efforts. Plasmodium falciparum surface protein Pfs47 is critical in the parasite’s survival by manipulating the vector’s immune system hence a promising target for blocking transmission in the mosquito. This study aimed to examine the genetic diversity, haplotype distribution, and population structure of Pfs47 and its implications on malaria infections in endemic lowlands in Western Kenya. Cross-sectional mass blood screening was conducted in malaria endemic regions in the lowlands of Western Kenya: Homa Bay, Kombewa, and Chulaimbo. Dried blood spots and slide smears were simultaneously collected in 2018 and 2019. DNA was extracted using Chelex method from microscopic Plasmodium falciparum positive samples and used to genotype Pfs47 using polymerase chain reaction (PCR) and DNA sequencing. Thirteen observed haplotypes of the Pfs47 gene were circulating in Western Kenya. Population-wise, haplotype diversity ranged from 0.69 to 0.77 and the nucleotide diversity 0.10 to 0.12 across all sites. All the study sites displayed negative Tajima’s D values although not significant. However, the negative and significant Fu’s Fs statistical values were observed across all the study sites, suggesting population expansion or positive selection. Overall genetic differentiation index was not significant (FST = -0.00891, P > 0.05) among parasite populations. All Nm values revealed a considerable gene flow in these populations. These results could have important implications for the persistence of high levels of malaria transmission and should be considered when designing potential targeted control interventions.
- Subjects :
- Plasmodium
Heredity
Protozoan Proteins
Population genetics
Nucleotide diversity
Geographical Locations
Medical Conditions
Gene Frequency
Genotype
Medicine and Health Sciences
2.2 Factors relating to the physical environment
Malaria, Falciparum
Aetiology
Protozoans
Genetics
education.field_of_study
Multidisciplinary
Membrane Glycoproteins
Ecology
biology
Malarial Parasites
Anopheles
Eukaryota
Genetic Mapping
Infectious Diseases
Medicine
Infection
Research Article
Falciparum
Ecological Metrics
General Science & Technology
Science
Population
Plasmodium falciparum
Rare Diseases
Parasite Groups
parasitic diseases
Parasitic Diseases
medicine
Humans
education
Evolutionary Biology
Genetic diversity
Population Biology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Genetic Variation
Species Diversity
Tropical Diseases
medicine.disease
biology.organism_classification
Kenya
Parasitic Protozoans
Malaria
Vector-Borne Diseases
Cross-Sectional Studies
Good Health and Well Being
Haplotypes
People and Places
Africa
Mutation
Parasitology
Apicomplexa
Population Genetics
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- PloS one, vol 16, iss 11, PLoS ONE, Vol 16, Iss 11, p e0260434 (2021), PLoS ONE
- Accession number :
- edsair.doi.dedup.....8d4a0b436cb7ecb566098c3767033b64