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Improvement of the in vitro T cell proliferation assay by a modified method that separates the antigen recognition and IL-2-dependent steps
- Source :
- Journal of Immunological Methods. 99:221-228
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- T cell activation is commonly assayed in vitro by measuring the proliferative response of primed cells to an antigenic stimulus. We have modified the conventional form of this assay by dividing up the response into two stages. During the first stage, antigen drives the specific expression of IL-2 receptor expression. This phase is carried out in the presence of homologous mouse serum, in order to reduce non-specific responses to a minimum. During the second phase, proliferation of these activated T cells is driven by the addition of excess exogenous IL-2. This modified form of proliferatiion assay significantly increased the signal to noise ratio which can be attained, and is of particular value when looking at the T cell response to weak (e.g., cross-reactive) antigens, or low concentrations of antigen.
- Subjects :
- Interleukin 2
T-Lymphocytes
Receptor expression
T cell
Immunology
Receptors, Antigen, T-Cell
Biology
Lymphocyte Activation
Mice
Antigen
Cell surface receptor
medicine
Animals
Insulin
Immunology and Allergy
Antigens
Receptors, Immunologic
Cells, Cultured
Cell growth
Lymphokine
Receptors, Interleukin-2
T lymphocyte
Molecular biology
medicine.anatomical_structure
Interleukin-2
Lymph Nodes
medicine.drug
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- Journal of Immunological Methods
- Accession number :
- edsair.doi.dedup.....8d43f1263f3ffec272582a1ba5494e31