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Polysialic Acid Acute Depletion Induces Structural Plasticity in Interneurons and Impairs the Excitation/Inhibition Balance in Medial Prefrontal Cortex Organotypic Cultures
- Source :
- Frontiers in Cellular Neuroscience
- Publication Year :
- 2016
- Publisher :
- Frontiers Media SA, 2016.
-
Abstract
- The structure and function of the medial prefrontal cortex (mPFC) is affected in several neuropsychiatric disorders, including schizophrenia and major depression. Recent studies suggest that imbalances between excitatory and inhibitory activity (E/I) may be responsible for this cortical dysfunction and, therefore, may underlie the core symptoms of these diseases. This E/I imbalance seems to be correlated with alterations in the plasticity of interneurons but there is still scarce information on the mechanisms that may link these phenomena. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) is a good candidate, because it modulates the neuronal plasticity of interneurons and its expression is altered in schizophrenia and major depression. To address this question, we have developed an in vitro model using mPFC organotypic cultures of transgenic mice displaying fluorescent spiny interneurons. After enzymatic depletion of PSA, the spine density of interneurons, the number of synaptic puncta surrounding pyramidal neuron somata and the E/I ratio were strongly affected. These results point to the polysialylation of NCAM as an important factor in the maintenance of E/I balance and the structural plasticity of interneurons. This may be particularly relevant for better understanding the etiology of schizophrenia and major depression.
- Subjects :
- 0301 basic medicine
Genetically modified mouse
PSA-NCAM
neuronal structural plasticity
Inhibitory postsynaptic potential
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
E/I balance
Neuroplasticity
medicine
Prefrontal cortex
Original Research
Polysialic acid
musculoskeletal, neural, and ocular physiology
medicine.disease
schizophrenia
mPFC cultures
030104 developmental biology
nervous system
Schizophrenia
Excitatory postsynaptic potential
Neural cell adhesion molecule
major depression
Psychology
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 16625102
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular Neuroscience
- Accession number :
- edsair.doi.dedup.....8d34254b81c769de7d8fb12b6c140932