Back to Search
Start Over
Towards a Molecular Definition of Enterohemorrhagic Escherichia coli (EHEC): Detection of Genes Located on O Island 57 as Markers To Distinguish EHEC from Closely Related Enteropathogenic E. coli Strains
- Source :
- Journal of Clinical Microbiology. 51:1083-1088
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- Among strains of Shiga-toxin (Stx) producing Escherichia coli (STEC), seven serogroups (O26, O45, O103, O111, O121, O145, and O157) are associated with severe clinical illness in humans. These strains are also called enterohemorrhagic E. coli (EHEC), and the development of methods for their reliable detection from food has been challenging thus far. PCR detection of major EHEC virulence genes stx 1 , stx 2 , eae , and O-serogroup-specific genes is useful but does not identify EHEC strains specifically. Searching for the presence of additional genes issued from E. coli O157:H7 genomic islands OI-122 and OI-71 increases the specificity but does not clearly discriminate EHEC from enteropathogenic E. coli (EPEC) strains. Here, we identified two putative genes, called Z2098 and Z2099, from the genomic island OI-57 that were closely associated with EHEC and their stx -negative derivative strains (87% for Z2098 and 91% for Z2099). Z2098 and Z2099 were rarely found in EPEC (10% for Z2098 and 12% for Z2099), STEC (2 and 15%), and apathogenic E. coli (1% each) strains. Our findings indicate that Z2098 and Z2099 are useful genetic markers for a more targeted diagnosis of typical EHEC and new emerging EHEC strains.
- Subjects :
- Microbiology (medical)
Genomic Islands
Virulence Factors
Virulence
Biology
medicine.disease_cause
Sensitivity and Specificity
Microbiology
Enteropathogenic Escherichia coli
fluids and secretions
STX2
hemic and lymphatic diseases
Genomic island
medicine
Animals
Humans
Gene
Escherichia coli
Escherichia coli Infections
Intimin
Bacteriological Techniques
Bacteriology
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Molecular Diagnostic Techniques
Genes, Bacterial
Genetic marker
Enterohemorrhagic Escherichia coli
bacteria
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....8d3162ad72c7312ec1c4a6f9e8153741