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A versatile method for the preparation of particle-loaded microbubbles for multimodality imaging and targeted drug delivery
- Source :
- Drug Delivery and Translational Research
- Publisher :
- Springer Nature
-
Abstract
- Microbubbles are currently in clinical use as ultrasound contrast agents and under active investigation as mediators of ultrasound therapy. To improve the theranostic potential of microbubbles, nanoparticles can be attached to the bubble shell for imaging, targeting and/or enhancement of acoustic response. Existing methods for fabricating particle-loaded bubbles, however, require the use of polymers, oil layers or chemical reactions for particle incorporation; embed/attach the particles that can reduce echogenicity; impair biocompatibility; and/or involve multiple processing steps. Here, we describe a simple method to embed nanoparticles in a phospholipid-coated microbubble formulation that overcomes these limitations. Magnetic nanoparticles are used to demonstrate the method with a range of different microbubble formulations. The size distribution and yield of microbubbles are shown to be unaffected by the addition of the particles. We further show that the microbubbles can be retained against flow using a permanent magnet, can be visualised by both ultrasound and magnetic resonance imaging (MRI) and can be used to transfect SH-SY5Y cells with fluorescent small interfering RNA under the application of a magnetic field and ultrasound field. Electronic supplementary material The online version of this article (doi:10.1007/s13346-017-0366-7) contains supplementary material, which is available to authorized users.
- Subjects :
- Materials science
Magnetic
Drug Compounding
Contrast Media
Pharmaceutical Science
Nanoparticle
Nanotechnology
02 engineering and technology
010402 general chemistry
01 natural sciences
Contrast agents
Drug Delivery Systems
Microscopy, Electron, Transmission
Cell Line, Tumor
Ultrasound
Methods Article
Humans
Particle Size
RNA, Small Interfering
Magnetite Nanoparticles
Phospholipids
Fluorescent Dyes
Ultrasonography
Targeting
Microbubbles
business.industry
fungi
Echogenicity
food and beverages
021001 nanoscience & nanotechnology
Magnetic Resonance Imaging
0104 chemical sciences
Microscopy, Fluorescence
Targeted drug delivery
Drug delivery
Particle
Magnetic nanoparticles
0210 nano-technology
business
Subjects
Details
- Language :
- English
- ISSN :
- 2190393X
- Database :
- OpenAIRE
- Journal :
- Drug Delivery and Translational Research
- Accession number :
- edsair.doi.dedup.....8d2698cb191a543953aec81eaa5f6408
- Full Text :
- https://doi.org/10.1007/s13346-017-0366-7