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A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis
- Source :
- Nature, Nature; Vol 494
- Publication Year :
- 2013
-
Abstract
- Experience from different fields of life sciences suggests that accessible, complete reference maps of the components of the system under study are highly beneficial research tools. Examples of such maps include libraries of the spectroscopic properties of molecules, or databases of drug structures in analytical or forensic chemistry. Such maps, and methods to navigate them, constitute reliable assays to probe any sample for the presence and amount of molecules contained in the map. So far, attempts to generate such maps for any proteome have failed to reach complete proteome coverage. Here we use a strategy based on high-throughput peptide synthesis and mass spectrometry to generate an almost complete reference map (97% of the genome-predicted proteins) of the Saccharomyces cerevisiae proteome. We generated two versions of this mass-spectrometric map, one supporting discovery-driven (shotgun) and the other supporting hypothesis-driven (targeted) proteomic measurements. Together, the two versions of the map constitute a complete set of proteomic assays to support most studies performed with contemporary proteomic technologies. To show the utility of the maps, we applied them to a protein quantitative trait locus (QTL) analysis, which requires precise measurement of the same set of peptides over a large number of samples. Protein measurements over 78 S. cerevisiae strains revealed a complex relationship between independent genetic loci, influencing the levels of related proteins. Our results suggest that selective pressure favours the acquisition of sets of polymorphisms that adapt protein levels but also maintain the stoichiometry of functionally related pathway members.
- Subjects :
- Proteomics
Saccharomyces cerevisiae Proteins
Proteome
Saccharomyces cerevisiae
Quantitative Trait Loci
Shotgun
Computational biology
Quantitative trait locus
Mass Spectrometry
Set (abstract data type)
03 medical and health sciences
Peptide Library
Reference Values
Selection, Genetic
Peptide library
030304 developmental biology
Genetics
0303 health sciences
Multidisciplinary
Polymorphism, Genetic
biology
030302 biochemistry & molecular biology
biology.organism_classification
Mass spectrometric
Subjects
Details
- Language :
- English
- ISSN :
- 00280836
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....8d23224ad9ad9abeed9d4f3e937d9b6f
- Full Text :
- https://doi.org/10.1038/nature11835