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Effects of nucleotide changes on the ability of hepatitis delta virus to transcribe, process, and accumulate unit-length, circular RNA
- Source :
- Journal of Virology. 71:5408-5414
- Publication Year :
- 1997
- Publisher :
- American Society for Microbiology, 1997.
-
Abstract
- The circular RNA genome of hepatitis delta virus (HDV) can fold into an unbranched rodlike structure. We mutagenized the two ends of this structure and assayed the effects on the ability of the genomes to replicate and accumulate processed RNA transcripts in transfected cells. The top end, defined as that nearest to the 5' end of the putative mRNA for delta antigen, was much more sensitive than the other end, defined as the bottom. Most of the 22 mutants made at the bottom were able to accumulate RNA as well as the wild type. For deletions extending as close as 2 nucleotides (nt) from the predicted domains needed for the two ribozymes, the accumulation levels dropped to
- Subjects :
- Time Factors
Transcription, Genetic
Molecular Sequence Data
Immunology
Mutant
Microbiology
Cell Line
Terminal loop
Open Reading Frames
Transcription (biology)
Circular RNA
Virology
Animals
Humans
Hepatitis delta Antigens
Messenger RNA
Base Sequence
biology
Hepatitis Antigens
Ribozyme
Wild type
RNA
Molecular biology
Mutagenesis
Insect Science
COS Cells
biology.protein
Nucleic Acid Conformation
RNA, Viral
Hepatitis Delta Virus
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....8d1e1eaa740ff0c4f5f7b611f9c65de2