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Identification of a 43-kDa outer membrane protein of Fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other Fusobacterium species
- Source :
- Research in veterinary science. 95(1)
- Publication Year :
- 2012
-
Abstract
- A pair of primers was designed in an attempt to amplify outer membrane protein (OMP) gene of Fusobacterium necrophorum based on nucleotide sequence of the OMP of Fusobacterium nucleatum. Further analysis was performed to characterize its molecular properties and phylogeny in the genus Fusobacterium. We identified a predicated 43kDa outer membrane protein (43K OMP) in F. necrophorum, which showed the same properties as other pore-forming proteins of Gram-negative anaerobic bacteria according to analysis of signal peptide, AT-rich, membrane-spanning region and conserved motifs. The predicated 43K OMP exhibited 70.22%, 62.04%, 56.75%, 58.72%, 51.59%, 31.49% and 50.26% amino acid identity with the OMPs of F. nucleatum, Fusobacterium varium, Fusobacterium ucerans, Fusobacterium periodonticum, Fusobacterium mortiferum, Fusobacterium gonidiaformans and F. necrophorum (hypothetical protein), respectively. 11 common conserved domains and 10 common variable domains were found among the 45 aligned OMPs of Fusobacterium species. Distributions of the conserved and variable domains were highly associated with predicted membrane-spanning regions, cell surface exposed regions and B-cell epitope regions. Phylogenetic analysis revealed the predicated 43K OMP of F. necrophorum was closely related with the OMPs from F. nucleatum and F. periodonticum. These data will increase understanding of pathogenesis and genetic evolution of F. necrophorum.
- Subjects :
- DNA, Bacterial
Hypothetical protein
ved/biology.organism_classification_rank.species
Amino Acid Motifs
Molecular Sequence Data
Protein Sorting Signals
Polymerase Chain Reaction
Microbiology
Fusobacterium periodonticum
Fusobacterium mortiferum
stomatognathic system
Fusobacterium necrophorum
Amino Acid Sequence
Cloning, Molecular
Phylogeny
General Veterinary
biology
Base Sequence
ved/biology
Sequence Analysis, DNA
biology.organism_classification
Fusobacterium gonidiaformans
stomatognathic diseases
Fusobacterium
Anaerobic bacteria
Fusobacterium nucleatum
Sequence Alignment
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 15322661
- Volume :
- 95
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Research in veterinary science
- Accession number :
- edsair.doi.dedup.....8d176563c44ecb4a5d80504273bc7e7f