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Identification of Novel Inhibitors of Auxin-Induced Ca 2+ Signaling via a Plant-Based Chemical Screen
- Source :
- Plant Physiology
- Publication Year :
- 2019
-
Abstract
- Many signal perception mechanisms are connected to Ca2+-based second messenger signaling to modulate specific cellular responses. The well-characterized plant hormone auxin elicits a very rapid Ca2+ signal. However, the cellular targets of auxin-induced Ca2+ are largely unknown. Here, we screened a biologically annotated chemical library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions to better understand the molecular mechanism of auxin-induced Ca2+ and to explore the physiological relevance of Ca2+ in auxin signal transduction. Using this approach, we defined a set of diverse, small molecules that interfere with auxin-induced Ca2+ entry. Based on annotated biological activities of the hit molecules, we found that auxin-induced Ca2+ signaling is, among others, highly sensitive to disruption of membrane proton gradients and the mammalian Ca2+ channel inhibitor bepridil. Whereas protonophores nonselectively inhibited auxin-induced and osmotic stress-induced Ca2+ signals, bepridil specifically inhibited auxin-induced Ca2+ We found evidence that bepridil severely alters vacuolar morphology and antagonized auxin-induced vacuolar remodeling. Further exploration of this plant-tailored collection of inhibitors will lead to a better understanding of auxin-induced Ca2+ entry and its relevance for auxin responses.
- Subjects :
- 0106 biological sciences
chemistry.chemical_classification
biology
Physiology
fungi
food and beverages
Plant Science
biology.organism_classification
01 natural sciences
Small molecule
Chemical library
Cell biology
chemistry.chemical_compound
chemistry
Auxin
Cell culture
Arabidopsis
Bepridil
Second messenger system
Genetics
medicine
heterocyclic compounds
Plant hormone
010606 plant biology & botany
medicine.drug
Subjects
Details
- ISSN :
- 00320889
- Database :
- OpenAIRE
- Journal :
- Plant Physiology
- Accession number :
- edsair.doi.dedup.....8d0fa0bee01d3e5efb3e7d4fd642d1f7
- Full Text :
- https://doi.org/10.1104/pp.18.01393