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Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
- Source :
- Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018), Scientific Reports, Scientific Reports, 8(1). NLM (Medline)
- Publication Year :
- 2018
- Publisher :
- Nature Portfolio, 2018.
-
Abstract
- Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that these effects are accompanied by changes of DNA methylation possibly inherited by subsequent generations. We assessed DNA methylation in F1 embryos (5.5 hours post fertilization) with whole genome bisulfite sequencing following parental exposure to 8.7 mGy/h for 27 days and found 5658 differentially methylated regions (DMRs). DMRs were predominantly located at known regulatory regions, such as gene promoters and enhancers. Pathway analysis indicated the involvement of DMRs related to similar pathways found with gene expression analysis, such as development, apoptosis and cancers, which could be linked to previous observed developmental defects and genomic instability in the offspring. Follow up of 19 F1 DMRs in F2 and F3 embryos revealed persistent effects up to the F3 generation at 5 regions. These results indicate that ionizing radiation related effects in offspring can be linked to DNA methylation changes that partly can persist over generations. Monitoring DNA methylation could serve as a biomarker to provide an indication of ancestral exposures to ionizing radiation.
- Subjects :
- 0301 basic medicine
Genome instability
Embryo, Nonmammalian
Gametogenesis
Epigenesis, Genetic
0302 clinical medicine
Transgenerational Effects
Radiation, Ionizing
zebra fish
Zebrafish
Regulation of gene expression
DNA methylation
Multidisciplinary
biology
Reproduction
article
apoptosis
Gene Expression Regulation, Developmental
biological marker
Cell biology
030220 oncology & carcinogenesis
Medicine
ionizing radiation
enhancer region
Differentially Expressed Genes (DEGs)
DNA damage
Offspring
congenital malformation
Science
animal experiment
embryo
WGBS Data
malignant neoplasm
progeny
Article
Genomic Instability
03 medical and health sciences
promoter region
follow up
bisulfite
Animals
controlled study
Differentially Methylated Regions (DMRs)
Enhancer
nonhuman
DNA Methylation
Zebrafish Proteins
genomic instability
biology.organism_classification
Whole-genome Bisulfite Sequencing (WGBS)
monitoring
030104 developmental biology
Differentially methylated regions
fertilization
gene expression
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8d0f1119196ba23854ac4c3b9fc14945